By: Firescifly . com

(Fireflysci.com: Farmingdale, NY) -- For several decades, spectrophotometry has been used in microbiology, clinical chemistry, the pharmaceutical industry, and other research applications.

Periodic verification of the performance of spectrophotometers is required by ISO 9001, good laboratory practice (GLP), good manufacturing practice (GMP), standard operating procedures (SOP) in the pharmaceutical industry, EP, DAB, USP (pharmacopeias), and other applicable standards and regulations.

Periodic verification of performance of spectrophotometers typically includes testing the resolution, wavelength accuracy, photometric accuracy, and stray-light affect. Traditionally, the calibration of spectrophotometers has been performed with a combination of liquid filters and solid-state filters. The solid-state filters only allowed the checking of photometric accuracy and wavelength accuracy usually within the 450 nm – 630 nm range. The liquid filters allow checking the photometric accuracy, wavelength accuracy, stray-light affect, and photometric resolution usually within the 200 nm – 660 nm range.

Liquid and solid-state filters are calibrated by the filter manufacturers, and the corresponding filters’ performance certificates are supplied to customers.

Carl Zeiss Microscopy’s picture

By: Carl Zeiss Microscopy

(Carl Zeiss MicroImaging Inc.: Thornwood, NY) -- The Primo Star iLED microscope now available with three additional fluorescence attachments that allow users to work with different dyes such as FITC, Dapi, and Rhodamine/Cy 3. This permits the use of the LED-based microscope for single fluorescence applications in training, routine procedures, and basic research. It is particularly robust and easy to use, and features an outstanding price-performance ratio, to which the long-lasting, inexpensive and energy-saving LED light source makes a valuable contribution. 

For training in particular, fluorescence excitation through LED illumination is beneficial as there are no warm-up or cool-down times, unlike fluorescence excitation using mercury vapor lamps. Furthermore, the excitation lamps no longer need to be readjusted, enabling the user to start working as soon as the light source is turned on. The iLED fluorescence attachment comes with practical eyecups, making it possible to perform fluorescence microscopy without a darkroom—in an auditorium, for example. A lever is used to alternate between brightfield and fluorescence microscopy. 

The Primo Star iLED was originally developed with the Foundation for Innovative New Diagnostics for a fast and reliable LED-fluorescence-based testing of tuberculosis. It has been on the market since October 2008. 

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